Microprojectile and Agrobacterium-Mediated Transformation of Pioneer Elm
Autor: | Mark Bolyard, Ravindra Hajela, Mariam Sticklen |
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Rok vydání: | 1991 |
Předmět: | |
Zdroj: | Arboriculture & Urban Forestry. 17:34-37 |
ISSN: | 2155-0778 1935-5297 |
DOI: | 10.48044/jauf.1991.011 |
Popis: | We have transformed internode sections of the hybrid elm cultivar "Pioneer" to assess the possibilities of introducing significant genes into elms. Foreign DNA encoding beta-glucuronidase (GUS) was introduced into internode sections using microprojectile- or Agrobacterium tumefaciensmediated transformation. Positive signals were detected by Southern analysis. Also, some enzymatic activity was detected in Agrobacterium transformed cultures using a fluorogenic assay. |
Databáze: | OpenAIRE |
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