Microprojectile and Agrobacterium-Mediated Transformation of Pioneer Elm

Autor: Mark Bolyard, Ravindra Hajela, Mariam Sticklen
Rok vydání: 1991
Předmět:
Zdroj: Arboriculture & Urban Forestry. 17:34-37
ISSN: 2155-0778
1935-5297
DOI: 10.48044/jauf.1991.011
Popis: We have transformed internode sections of the hybrid elm cultivar "Pioneer" to assess the possibilities of introducing significant genes into elms. Foreign DNA encoding beta-glucuronidase (GUS) was introduced into internode sections using microprojectile- or Agrobacterium tumefaciensmediated transformation. Positive signals were detected by Southern analysis. Also, some enzymatic activity was detected in Agrobacterium transformed cultures using a fluorogenic assay.
Databáze: OpenAIRE