| Popis: |
Oncogenic Ras causes down‐regulation of the proapoptotic tumor suppressor gene Par‐4. Replenishment of the basal levels of Par‐4 results in inhibition of Ras‐inducible cellular transformation. Moreover, overexpression of Par‐4 (twofold to fourfold over basal levels) results in apoptosis of cells expressing oncogenic Ras. Par‐4 does not, on its own, induce apoptosis in immortalized or nontransformed cells. This chapter describes the key methods used for analysis of Par‐4 down‐regulation by oncogenic Ras, which can be extended to study most genes whose down‐regulation by oncogenic Ras is critical for oncogenic transformation and cell survival. |
