Abstract W P102: Predominant Activation of M2 Macrophages by Group-specific Component Protein-derived Macrophage Activating Factor in the Late Phase of Cerebral Ischemia may Contribute to Neurogenesis
| Autor: | Yoshitaka Kurashiki, Keiko T Kitazato, Kenji Shimada, Kenji Yagi, Yoshiteru Tada, Tomoya Kinouchi, Manabu Sumiyoshi, Takeshi Miyamoto, Junichiro Satomi, Teruyoshi Kageji, Yoshihiro Uto, Shinji Nagahiro |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Stroke. 46 |
| ISSN: | 1524-4628 0039-2499 |
| DOI: | 10.1161/str.46.suppl_1.wp102 |
| Popis: | Background and Purpose: Peripheral macrophages include M1- and M2 macrophages; they produce pro-inflammatory and anti-inflammatory cytokines, respectively. Inflammation produces group-specific component protein-derived macrophage activating factor (GcMAF). The role of M1- and M2 macrophages activated by GcMAF in the post-ischemic brain remains unclear. We hypothesized that the role of GcMAF after ischemia is time-dependently different and that the dominant activation of M2 macrophages facilitates brain repair. Methods: After subjecting 7-week-old male Wistar rats to 2-hr middle cerebral artery occlusion they were divided into a group injected for 7 days with GcMAF and a group serving as the vehicle control in the early- and late phase after ischemia induction. Results: Within 3 days of post-ischemia, the predominant increase in M1 macrophages was associated with expanded ischemic brain damage that was exacerbated by GcMAF. At the 7th post-ischemic day the infarct size was stabilized and M1 macrophages were decreased, although the higher expression in M1- than M2 macrophages persisted. Interestingly, GcMAF treatment from this point cleaned the infarct area, which was associated with phagocytosis via a predominant increase in microglia and M2 macrophages. The effect of GcMAF was abrogated by macrophage-depleting clodronate liposomes. Furthermore, the increase in M2 macrophages was associated with increased BrdU incorporation and the appearance of survival-related molecules and neurovascular unit, suggesting the promotion of neurogenesis. Conclusion: The activation of M2 macrophages by GcMAF in the late phase of cerebral ischemia may be associated with brain repair. |
| Databáze: | OpenAIRE |
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