The inhibitory role of clioquinol in the fibrillation of hen egg white lysozyme
| Autor: | Xudong Zhu, Haohao Wang, Yujie Liu, Fang Wu, Baoliang Ma |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fibrillation
Circular dichroism Chemistry Clioquinol General Physics and Astronomy 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Inhibitory postsynaptic potential 01 natural sciences Fluorescence Protein tertiary structure 0104 chemical sciences Congo red chemistry.chemical_compound medicine Biophysics Physical and Theoretical Chemistry medicine.symptom Lysozyme 0210 nano-technology medicine.drug |
| Zdroj: | Chemical Physics Letters. 779:138830 |
| ISSN: | 0009-2614 |
| DOI: | 10.1016/j.cplett.2021.138830 |
| Popis: | Here, we reported the role of clioquinol in amyloid aggregation of hen egg white lysozyme (HEWL) under stressful conditions by several biophysical methods including thioflavin-T (ThT) fluorescence, Congo red (CR), circular dichroism (CD) and transmission electron microscopy. Our work showed that CQ inhibited the fibril formation of HEWL and the inhibitory effects depended on the concentrations of CQ. Furthermore, fluorescence spectra were used to study the conformation changes of HEWL caused by CQ. These data revealed that the interactions of CQ with HEWL changed the tertiary structure and inhibited the hydrophobic exposure of HEWL. |
| Databáze: | OpenAIRE |
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