High-hydrostatic-pressure optical chamber system for cultivation and microscopic observation of deep-sea organisms

Autor:	Shuming Ye, Kaikai Lou, Yun Gai, Chengman Bao, Chunyue Jiang
Rok vydání:	2010
Předmět:	Microscope Ecology business.industry Decompression Hydrostatic pressure Mineralogy Aquatic Science Biology Oceanography Deep sea Pressure sensor law.invention Microscopic observation Optics law Sapphire business Ecology Evolution Behavior and Systematics
Zdroj:	Aquatic Biology. 11:157-162
ISSN:	1864-7790 1864-7782
DOI:	10.3354/ab00303
Popis:	We developed and tested a high-pressure optical chamber system for cultivation and mi- croscopic observation of deep-sea organisms. The system is composed of an optical chamber, a high- pressure pump, a pressure sensor and a microscope. The chamber has an observation cavity, 2 cultiva- tion cavities and 2 sapphire windows. The pump is employed for perfusion of culture medium and for increasing the pressure. The pressure sensor monitors the pressure in the chamber. The microscope is used for observing samples through the sapphire windows. In the future, the optical chamber system could be used alone for short-term research or be connected to a large high-pressure vessel to create a flow-through system for long-term research. Using the system, the swimming activity of Bosmina lon- girostris (Branchiopoda: Cladocera) was observed at different pressures. Swimming activity increased with increasing compression up to 30 MPa. During decompression, this activity reappeared when pressure decreased to 45 MPa and increased with further decreasing pressure.
Databáze:	OpenAIRE
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