Appraisal of Sims applicability to boron studies in plants
| Autor: | Michel Thellier, Marie-Claire Verdus, Fabrice Lefebvre, David Gibouin, Maurice Demarty, Cedric Dérue, Camille Ripoll |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
0106 biological sciences
0303 health sciences Histology Stable isotope ratio Radiochemistry Analytical chemistry chemistry.chemical_element Isotopes of boron 01 natural sciences 03 medical and health sciences Medical Laboratory Technology Isotopic ratio chemistry Anatomy Boron Instrumentation 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Microscopy Research and Technique. 58:104-110 |
| ISSN: | 1059-910X |
| DOI: | 10.1002/jemt.10125 |
| Popis: | In the search for a new methodological approach applicable to the determination of the still poorly known primary role of boron in plant physiology, we have undertaken to appraise the potential of the SIMS method for the analytical imaging of the boron isotopes, 10B and 11B, at physiological concentrations in plants. With our own, CAMECA IMS4F SIMS ion analyser, and using O2+ as primary ions for the detection of B+ (plus 12C+ and 40Ca+) secondary ions, we have been able to map quantitatively the two boron isotopes in control and boron-enriched plants, to evaluate boron concentrations at the level of individual cells and to determine boron isotopic ratios. This provides the opportunity to carry out the simultaneous labeling and imaging of boron, using enrichment with the stable isotopes, 10B and 11B. The method has also the potential for the simultaneous, quantitative detection of the boron isotopes and of the borate-binding sites in plant cells. Microsc. Res. Tech. 58:104–110, 2002. © 2002 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text