A comparison of fluorescent SSCP and denaturing HPLC for high throughput mutation scanning

Autor:	C.F. Taylor, Graham R. Taylor, Lucy A. Ellis
Rok vydání:	2000
Předmět:	Absorbance Denaturing hplc Mutation (genetic algorithm) Genetics Mutation detection Single-strand conformation polymorphism Reversed-phase chromatography Amplicon Biology Fluorescence Molecular biology Genetics (clinical)
Zdroj:	Human Mutation. 15:556-564
ISSN:	1098-1004 1059-7794
DOI:	10.1002/1098-1004(200006)15:6<556::aid-humu7>3.0.co;2-c
Popis:	We examined 67 different mutations in 16 different amplicons in a comparison of mutation detection by fluorescent single strand conformation polymorphism (F-SSCP) and by denaturing HPLC (DHPLC). F-SSCP was used to analyze fluorescent amplicons with internal size standards and automated fragment analysis (GeneScan, PE Applied Biosystems, Foster City, CA). In DHPLC, unlabelled amplicons were analyzed by reverse phase HPLC with fragment detection by absorbance at 260nm. Both methods had high sensitivity (95–100%) and specificity (100%). Overall, F-SSCP with external temperature control was the more sensitive method, but DHPLC was particularly useful for the rapid analysis of novel fragments. Hum Mutat 15:556–564, 2000. © 2000 Wiley-Liss, Inc.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4b90fe7a36941072db93c588a15f63be https://doi.org/10.1002/1098-1004(200006)15:6<556::aid-humu7>3.0.co Zobrazit plný text záznamu Plný text