Growth Factors and Gene Expression in Silica-Induced Fibrogenesis and Carcinogenesis
| Autor: | Umberto Saffiotti, Alan D. Knapton, A. Olufemi Williams |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Lung biology Chemistry Mesenchymal stem cell Public Health Environmental and Occupational Health medicine.disease_cause Molecular biology medicine.anatomical_structure Cell culture medicine Extracellular biology.protein Antibody Carcinogenesis Intracellular Transforming growth factor |
| Zdroj: | Applied Occupational and Environmental Hygiene. 10:1089-1098 |
| ISSN: | 1521-0898 1047-322X |
| Popis: | Transforming growth factor β1 (TGF-β1), ras, and p53 were studied in the rat model for silica-induced fibrogenesis and carcinogenesis, and in the FRLE cell line, derived from fetal rat alveolar pneumocytes. In Fischer-344 rats of both sexes, following single intratracheal instillation of quartz, mesenchymal and epithelial lung reactions were examined immunohistochemically for reactivity to (1) intracellular mature TGF-β1, localized in fibroblasts and macrophages at the periphery of silicotic granulomas adjacent to hyperplastic alveolar type II (HP-TII) cells; (2) extracellular mature TGF-β1, localized in connective tissue matrix adjacent to HP-TII cells; and (3) TGF-β1 precursor/latency-associated peptide (LAP), localized intracellularly in HP-TII cells and in adenomas, but not in carcinomas. Immunoreactivity to the three antibodies for TGF-β1 was first detected between 10 and 15 days from treatment. Hematite-treated controls showed no reactivity to TGF-β1. Production of TGF-β1 by HP-TII cells is... |
| Databáze: | OpenAIRE |
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