Photoresist-based integration of enzyme functionality into MEMS
| Autor: | Ilkka Lähdesmäki, Babak A. Parviz, Nicole K. Thomas |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microelectromechanical systems
Fluorescence-lifetime imaging microscopy Materials science Fabrication biology Nanotechnology Photoresist Condensed Matter Physics Electronic Optical and Magnetic Materials Hardware and Architecture biology.protein Glucose oxidase Lactate oxidase Electrical and Electronic Engineering Biosensor Microfabrication |
| Zdroj: | Microsystem Technologies. 17:1505-1510 |
| ISSN: | 1432-1858 0946-7076 |
| Popis: | We demonstrate the direct immobilization of glucose oxidase and lactate oxidase onto photoresists commonly used in microfabrication. The method allows for a cost-effective, facile inclusion of enzyme functionality into novel MEMS devices because it does not require any chemical or physical pre-treatment of surfaces, and it is largely compatible with existing fabrication technologies. We used fluorescence imaging and absorbance spectrometry to confirm attachment of the enzymes onto the photoresists and to determine their activity. In addition, the procedure was used to successfully integrate enzyme functionality into a photoresist-based biosensor. This further demonstrates the effectiveness of the approach, and opens a path towards other novel applications in MEMS research. |
| Databáze: | OpenAIRE |
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