Magnetic beads based colorimetric detection of mercuric ion
| Autor: | Hanyin Wu, Liangqia Guo, Liangshuang Zhong, Jing Wang, Guonan Chen, Xijing Huang, Yanlin Hao, Qingquan Guo, Tianran Lin, FengFu Fu |
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| Rok vydání: | 2014 |
| Předmět: |
Detection limit
Mercuric ion animal structures ABTS Chemistry Base pair Inorganic chemistry Metals and Alloys Deoxyribozyme Analytical chemistry Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Absorbance chemistry.chemical_compound Materials Chemistry Chelation Electrical and Electronic Engineering Instrumentation Hemin |
| Zdroj: | Sensors and Actuators B: Chemical. 191:600-604 |
| ISSN: | 0925-4005 |
| Popis: | A simple and sensitive DNAzyme-based colorimetric sensor for detecting Hg2+ based on T–Hg2+-T base pair and magnetic beads (MBs) is proposed. In the presence of Hg2+, capture probes (CPs) immobilized on MBs are hybridized with the signal probes (SPs) consisting of G-quadruplex sequences with the help of T–Hg2+-T base pairs. The SPs are separated by MBs and used to bind hemin for the formation of catalytically active hemin/G-quadruplex DNAzymes, which catalyzes the H2O2-mediated oxidation of ABTS to produce a blue-green-colored product. A linear relationship between the absorbance and Hg2+ concentration is observed over a range of 10–200 nM with a limit of detection 5 nM. Taking advantage of MBs separation/collection process, the sensing system can be regenerated by using EDTA as the chelator. |
| Databáze: | OpenAIRE |
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