Ginsenoside retinoblastoma 1 (Rb1) suppresses NO production and inducible nitric oxide synthase (iNOS) expression by inhibiting nuclear factor B (NF-B) activation in SW1353 chondrosarcoma cells
| Autor: | Zhijun Ge, Ruhua Chen, Keyun Shi, Jianzhong Jiang, Jiannong Jiang, Zhenhuan Jiang, Xinnan Gu, Yanping Zhao |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
biology medicine.diagnostic_test business.industry Retinoblastoma Pharmaceutical Science medicine.disease eye diseases Chondrocyte Nitric oxide synthase Ginseng IκBα chemistry.chemical_compound medicine.anatomical_structure Western blot Downregulation and upregulation chemistry Ginsenoside biology.protein medicine business |
| Zdroj: | African Journal of Pharmacy and Pharmacology. 7:2584-2590 |
| ISSN: | 1996-0816 |
| DOI: | 10.5897/ajpp12.138 |
| Popis: | Ginseng (Panax ginseng C.A. Mey) is commonly used to treat osteoarthritis (OA) in Chinese traditional medicine (TCM). In this study, we investigated whether ginsenoside retinoblastoma 1 (Rb1), an active component of ginseng, could regulate NO production in chondrocytes and its potential mechanisms of action. SW1353 cells were stimulated with IL-1β in the presence of different concentrations of ginsenoside Rb1. NO concentration was assessed by the Griess reaction. Expression of iNOS, degradation of Ii«Bα and nuclear translocation of NF-κB p65 were determined by Western blot. DNA binding activity of NF-κB complex was evaluated with Trans AM™ kit for p65. We found that ginsenoside Rb1 significantly decreased the NO production and iNOS protein expression in a concentration-dependent manner. Ginsenoside Rb1 markedly decreased the IκBα degradation and nuclear p65 levels, as well as inhibited the DNA binding activity of NF-κB complex. These results suggest that ginsenoside Rb1 inhibits IL-1β-induced NO production through downregulation of NF-κB-dependent iNOS expression in chondrocytes, and reveals potential mechanisms explaining the benefits of ginseng for OA treatment in TCM. Key words: Ginsenoside, retinoblastoma 1 (Rb1), NO, inducible nitric oxide synthase (iNOS), nuclear factor κB (NF-κB), chondrocyte, osteoarthritis. |
| Databáze: | OpenAIRE |
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