Amplification by the polymerase chain reaction of a specific target sequence in the gene coding forEscherichia coliverotoxin (VTe variant)
| Autor: | H. Lior, G. Wang, S.D. Tyler, W.M. Johnson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Genetics
biology Oligonucleotide Inverse polymerase chain reaction medicine.disease_cause biology.organism_classification Microbiology Enterobacteriaceae Molecular biology law.invention chemistry.chemical_compound chemistry law medicine Coding region Molecular Biology Escherichia coli Gene Polymerase chain reaction DNA |
| Zdroj: | FEMS Microbiology Letters. 84:227-230 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in the same coding regions for type 1, type 2, and type 2 variant E. coli verotoxins. This procedure will be a valuable adjunct to other DNA amplification techniques currently being used for molecular epidemiological studies of verotoxigenic E. coli. |
| Databáze: | OpenAIRE |
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