Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
| Autor: | Rastislav Mucha, Gabriela Štrkolcová, Eva Barbušinová, Marián Maďar, Jana Kačírová, Kristína Mravcová, Mária Goldová |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology 030231 tropical medicine Public Health Environmental and Occupational Health Cryptosporidium biology.organism_classification Staining technique Virology 030308 mycology & parasitology 03 medical and health sciences 0302 clinical medicine Cryptosporidium parvum parasitic diseases Elisa test Parasite hosting Protozoa Health risk Waste Management and Disposal Nested polymerase chain reaction Ecology Evolution Behavior and Systematics |
| Zdroj: | Annals of Agricultural and Environmental Medicine. 27:485-488 |
| ISSN: | 1898-2263 1232-1966 |
| DOI: | 10.26444/aaem/126619 |
| Popis: | Introduction and objectives The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Material and methods The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusions This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|