What Is the 'Areca' in 'Areca Nuts'? Extraction and Neuroactive Bioassay of Arecoline
| Autor: | Farid Sanai, Katherine E. S. Locock, Tim Bakas, Tina Hinton, Robin D. Allan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
biology
Traditional medicine Chemistry digestive oral and skin physiology 010401 analytical chemistry 05 social sciences food and beverages 050301 education General Chemistry Catechu Smooth muscle contraction biology.organism_classification 01 natural sciences 0104 chemical sciences Education Toxicology Atropine Smooth muscle Muscarinic acetylcholine receptor medicine Bioassay Arecoline 0503 education Areca medicine.drug |
| Zdroj: | Journal of Chemical Education. 93:197-201 |
| ISSN: | 1938-1328 0021-9584 |
| Popis: | A series of three practical sessions are designed to give students firsthand experience with the preparation of natural product extracts and assay using a live tissue preparation. Areca or betel nuts are the seeds from the fruit of the Areca catechu palm tree that is known to contain a number of pharmacologically active alkaloids. The principal of these is arecoline that makes up to 1% of the dry nut. Arecoline is a potent spasmogenic agent, causing smooth muscle contraction via muscarinic acetylcholine receptor (mAChR) activation. The first session involves the preparation of methanolic extracts from whole areca nuts and TLC for the qualitative identification of arecoline present in the extract. The second session utilizes the spasmodic effects of arecoline on smooth muscle to allow students to perform a live tissue bioassay using guinea-pig ileum. This response is subsequently blocked by the mAChR antagonist atropine to investigate the mechanism underlying the measured response. The final session gives ... |
| Databáze: | OpenAIRE |
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