Time-course phosphorylation of glucose by Saccharomyces carlsbergensis following alterations in cell membrane permeability
| Autor: | A. Tommasi, F. Melelli, G. M. Bisso |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Hexokinase Sugar phosphates biology Membrane permeability Aldolase A Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology Saccharomyces chemistry.chemical_compound Biochemistry chemistry biology.protein Fermentation Incubation Biotechnology Phosphofructokinase |
| Zdroj: | Biotechnology Letters. 11:669-672 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01025280 |
| Popis: | The correlation between release of sugar phosphates and the increase of membrane permeability was assessed in Saccharomyces carlsbergensis cells. The highest level of fructose-1,6-diphosphate,FDP, (35–40 mg/ml) was reached after 6h incubation at 35°C (65–70% permeabilized cells) while it was less than 1 mg/ml after 22 h incubation at 15 °C (only 10% permeabilized cells). Assessment of enzymatic activity of hexokinase (HK) phosphofructokinase (PFK) and aldolase (AL) during fermentation showed a higher leakage of both kinases in permeabilized cells than in intact ones. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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