Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis
| Autor: | Lindsey Devisscher, Hans Van Vlierberghe, Sanne Van Campenhout |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cirrhosis Common bile duct Bile duct business.industry Biliary cirrhosis Bile obstruction medicine.disease Gastroenterology 03 medical and health sciences Liver disease 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Cholestasis Internal medicine medicine 030211 gastroenterology & hepatology Ligation business |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493994199 |
| DOI: | 10.1007/978-1-4939-9420-5_15 |
| Popis: | Cholestatic liver disease covers a range of biliary disorders marked by an impaired bile duct flow. Various conditions can result in bile obstruction including choledocholithiasis, surgical trauma, and autoimmune disorders. Cholestatic liver disease can be mild but generally progresses to more severe conditions with increased hepatobiliary injury, cholangitis, and ultimately liver fibrosis and cirrhosis. An extensively used experimental model to investigate the pathophysiology of biliary cirrhosis and potential novel therapies is the common bile duct ligation in mice and rats. Common bile duct ligation induces the different stages of cholestatic-induced liver disease being cholestasis, subsequently accompanied by inflammation and finally liver fibrosis and cirrhosis. In this protocol, an outline of the surgical procedures to conduct common bile duct ligation in mice is provided. The major steps include the isolation of the common bile duct, followed by ligation and dissection. |
| Databáze: | OpenAIRE |
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