Comb interdigitated arrays (IDA) electrodes for more rapid and sensitive bead-based immunoassay
| Autor: | Chang Ming Li, Peter J. Hesketh, Jennifer H. Thomas, William R. Heineman, Brian Halsall, Sang Kyung Kim |
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| Rok vydání: | 2003 |
| Předmět: |
Detection limit
Materials science medicine.diagnostic_test business.industry Analytical chemistry nutritional and metabolic diseases Bead Signal hemic and lymphatic diseases Immunoassay visual_art Electrode Electrochemical immunoassay medicine visual_art.visual_art_medium Optoelectronics business Biosensor Signal amplification |
| Zdroj: | The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE. |
| DOI: | 10.1109/memsys.2003.1189779 |
| Popis: | This research is dedicated to the development of electrode arrays suitable for a more sensitive and faster electrochemical immunoassay system Signal amplification is achieved through coupling the redox cycling of interdigitated array (IDA) electrodes with an enzyme label, /spl beta/-galactosidase. Comb IDAs enhanced the signal 3 times higher than coplanar IDAs. A magnetic bead-based enzyme assay, as a simulation of an immunoassay, has been performed using the comb and coplanar IDAs for detection. Comb IDAs enabled positioning the beads and attached enzyme closer to the sensing electrodes (/spl sim/10 gm). Detection time was less than 1 min. and the limit of detection was 70 amole of /spl beta/-galactosidase. |
| Databáze: | OpenAIRE |
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