Diurnal Variations in the Activity of Phosphoenolpyruvate Carboxylase and NADP-Malic Enzyme During the Early Steps of Interaction between Glycine max and Bradyrhizobium japonicum

Autor:	Maria Geneva, George Ignatov, Yuliana Markovska, Valya Vassileva
Rok vydání:	2003
Předmět:	chemistry.chemical_classification biology Inoculation food and beverages Plant Science Horticulture NADP-malic enzyme biology.organism_classification Enzyme Biochemistry Symbiosis chemistry Botany Shoot Glycine Phosphoenolpyruvate carboxylase Bradyrhizobium japonicum
Zdroj:	Biologia plantarum. 46:399-403
ISSN:	1573-8264 0006-3134
DOI:	10.1023/a:1024386421350
Popis:	Two important enzyme in organic acid metabolism, phosphoenolpyruvate carboxylase (PEPC) and NADP-malic enzyme (NADP-ME), show marked diurnal rhythms in their activities during the establishment of the soybean - B. japonicum symbiosis. The pH of the nutrient solution changes in parallel with NADP-ME activity, being maximal during the night periods, whereas activity of PEPC was highest during the day periods. The results from the experiments with stem girdled plants indicated that the activity of root PEPC is modulated to a great extent by the supply of photosynthates from the shoots. It was also established that succinate application in the nutrient solution during inoculation altered significantly the pattern of assayed enzyme activities. Although our experiments did not reveal the precise mechanism of the involvement of root PEPC and NADP-ME in soybean response to inoculation with B. japonicum, they indicated the pattern of their activity during the first 72 h postinoculation which are critical for establishment and functioning of the symbiosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::44aee5b431901773d2365d7ed1499595 https://doi.org/10.1023/a:1024386421350 Zobrazit plný text záznamu Full text from SpringerLink