Cyclin D1 C.870G>a Polymorphism in Patients with Multiple Myeloma after Allogeneic Stem Cells Transplantation

Autor:	Antonio Pezzutto, Bernd Dörken, Ekaterina Slonova, Kathrin Rieger, Olga Blau, Igor Wolfgang Blau, Axel Nogai, Rimma Berenstein
Rok vydání:	2014
Předmět:	Oncology Sanger sequencing medicine.medical_specialty Immunology Chromosomal translocation Cell Biology Hematology Biology medicine.disease Biochemistry Transplantation symbols.namesake Cyclin D1 Internal medicine medicine symbols In patient Stem cell Multiple myeloma Cyclin
Zdroj:	Blood. 124:2576-2576
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v124.21.2576.2576
Popis:	Introduction: Over the last decade, the cyclin D1 (CCND1) c.870G>A polymorphism has been variously reported to confer risk for a large number of cancers. Deregulation of a D-group cyclin is a key feature of multiple myeloma (MM). Previously published meta-analysis shown a relationship between CCND1 c.870G>A and risk of t(11;14) in MM (Wienhold, 2013). Allogeneic stem cell transplantation (AlloSCT) is a potential curative treatment for MM patients. There are no data with regard to the role of CCND1 c.870G>A polymorphism in stem cells donors and outcome of transplantation in MM patients. Methods: In this study we developed an endonuclease restriction method to identify CCND1 c.870G>A polymorphism. Directly Sanger sequencing were used to confirm the results. Results: At first, we analyzed 130 MM patients and 100 healthy donors as a control. CCND1 c.870G>A polymorphism was strongly associated with the t(11;14)(q13;q32) (PA polymorphism 55 pairs of MM patients and their allogeneic stem cell donors, retrospectively. Interestingly, c.870G-genotype in related and unrelated donors was statistically correlated with poor outcome after AlloSCT (P Conclusion: Our data suggest the published data that constitutive genetic factor (CCND1 c.870 G>A polymorphism) is associated with a specific chromosomal translocation in patients with MM. Despite the fact that our group of patients after AlloSCT is small, we found a statistical association between the c.870G-genotype in donor and a poor prognosis after transplantation. Disclosures No relevant conflicts of interest to declare.
Databáze:	OpenAIRE
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