| Autor: |
Zheng Gang Zhu, Qu Cai, Lei Guo, Jian Fang Li, Min Yan, Jun Ji, Rong Juan Zhang, Ying Yan Yu, Xiao Bo Guo, Qiao Ming Zhi, Jin Ling Jiang, Jia Nian Zhang, Qin Long Gu, Xue Hua Chen, Bing Ya Liu, Jun Zhang |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of Gastroenterology and Hepatology. 26:1685-1690 |
| ISSN: |
0815-9319 |
| DOI: |
10.1111/j.1440-1746.2011.06808.x |
| Popis: |
Background and Aim: Gene silence of IRX1 tumor suppressor by promoter CpG methylation combined with loss of heterozygosity (LOH) has been identified in human gastric cancer. This study investigated the association between methylation of IRX1 and Helicobacter pylori infection in gastric mucosa tissues and cell line. Methods: IRX1 methylation was studied by methylation specific polymerase chain reaction (MSP) and bisulfate sequencing polymerase chain reaction (BSP) methods in gastric mucosa tissues from H. pylori-positive chronic gastritis patients or H. pylori-negative chronic gastritis patients. Promoter activity, methylation status and gene expressing level of IRX1 were evaluated by persistent infecting H. pylori on human gastric cells GES-1 in vitro. Electron microscopy was used to observe the effect of H. pylori infection on GES-1 gastric mucosa cells. Results: The methylation level of IRX1 promoter in H. pylori positive chronic gastritis and H. pylori negative chronic gastritis was 55.30% ± 13.17 versus 5.20% ± 6.31, respectively (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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