Determination of Structural and Morphological Parameters of Human Bulbar Conjunctiva from Optical Diffuse Reflectance Spectra
| Autor: | V. A. Firago, M. M. Kugeiko, S. A. Lisenko, A. Kubarko |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Observational error
Conjunctiva business.industry Chemistry Monte Carlo method Radiation Condensed Matter Physics 01 natural sciences eye diseases Spectral line 010309 optics 03 medical and health sciences 0302 clinical medicine Diffuse reflectance spectra medicine.anatomical_structure Optics 0103 physical sciences 030221 ophthalmology & optometry medicine sense organs Diffuse reflection Absorption (electromagnetic radiation) business Spectroscopy |
| Zdroj: | Journal of Applied Spectroscopy. 83:617-626 |
| ISSN: | 1573-8647 0021-9037 |
| DOI: | 10.1007/s10812-016-0337-7 |
| Popis: | We have developed a method for on-the-fl y retrieval of the volume concentration of blood vessels, the average diameter of the blood vessels, the blood oxygenation level, and the molar concentrations of chromophores in the bulbar conjunctiva from its diffuse reflectance spectra, measured when the radiation delivery and detection channels are spatially separated. The relationship between the diffuse reflectance spectrum of the conjunctiva and its unknown parameters is described in terms of an analytical model, constructed on the basis of a highly accurate approximation analog of the Monte Carlo method. We have studied the effect of localization of hemoglobin in erythrocytes and localization of erythrocytes in the blood vessels on the power of the retrieval of structural and morphological parameters for the conjunctiva. We developed a device for obtaining video images of the conjunctiva and contactless measurements of its diffuse reflectance spectrum. By comparing simulated diffuse reflectance spectra of the conjunctiva with the experimental measurements, we established a set of chromophores which must be taken into account in the model for reproducing the experimental data within the measurement error. We observed absorption bands for neuroglobin in the experimental spectra, and provided a theoretical basis for the possibility of determining its absolute concentrations in the conjunctiva. We have shown that our method can detect low bilirubin concentrations in blood. |
| Databáze: | OpenAIRE |
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