Two Distinct Types of Aspartic Proteases in the Filarial Parasite Brugia malayi: Molecular Cloning and Tissue Distribution

Autor:	Hideshi Inoue, Hiroyuki Nomura, Kenji Takahashi, Senarath B. P. Athauda
Rok vydání:	2003
Předmět:	chemistry.chemical_classification biology Phylogenetic tree General Medicine Molecular cloning biology.organism_classification Molecular biology General Biochemistry Genetics and Molecular Biology Brugia malayi Amino acid Nematode chemistry Biochemistry Reproductive system Peptide sequence Pathogen
Zdroj:	Biomedical Research. 24:269-276
ISSN:	1880-313X 0388-6107
DOI:	10.2220/biomedres.24.269
Popis:	The cDNAs coding for two distinct types of aspartic proteases, Bm-ASP-1 and Bm-ASP-2, were cloned from the parasitic nematode Brugia malayi, an important pathogen of human lymphatic filariasis, and their amino acid sequences (393 and 452 residues, respectively, as prepro forms) were deduced. Bm-ASP-1 and Bm-ASP-2 are 29% identical with each other in amino acid sequence and phylogenetic analysis indicated that Bm-ASP-1 and Bm-ASP-2 belong to two different subfamilies of aspartic proteases of nematodes. Immunohistochemical analysis indicated that Bm-ASP-1 is localized in the intestine and esophagus, while Bm-ASP-2 is distributed more ubiquitously in various tissues including intestine, esophagus, body wall muscle and reproductive system, suggesting their different roles in vivo.
Databáze:	OpenAIRE
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