TESTOSTERONE PRETREATMENT ENHANCES CADMIUM-INDUCED INCREASES IN c-myc AND c-jun TRANSCRIPT LEVELS IN L6 MYOBLASTS

Autor:	William E. Achanzar, James F. Hochadel, Michael P. Waalkes, Hideaki Shimada
Rok vydání:	1999
Předmět:	inorganic chemicals Cadmium Messenger RNA medicine.medical_specialty medicine.medical_treatment c-jun chemistry.chemical_element Biology Toxicology Pollution Steroid Endocrinology chemistry Internal medicine medicine Metallothionein Testosterone Carcinogen Hormone
Zdroj:	Toxic Substance Mechanisms. 18:129-137
ISSN:	1076-9188
DOI:	10.1080/107691899229098
Popis:	Cadmium is a known carcinogen that often targets male reproductive tissues. In many cells cadmium induces expression of the proto-oncogenes c-myc and c-jun. Several steroid hormones can modify the effects of cadmium, including testosterone. Thus, we studied the effects of testosterone on cadmium toxicity or c-myc and c-jun expression in L6 cells. Testosterone at non-toxic doses (0-10 muM) did not modify either cadmium-induced cytotoxicity or expression of metallothionein, a protein involved in acquired cadmium tolerance. Cadmium (5 muM) increased c-myc and c-jun m RNA levels by 3.1-fold and 2.8-fold of control, respectively. Testosterone alone (10 muM) decreased basal levels of c-myc transcript but did not affect c-jun. Despite reducing transcript levels by itself, testosterone pretreatment increased cadmium-induced c-myc transcript accumulation to 5-fold over control. Testosterone pretreatment also enhanced cadmium induction of c-jun mRNA accumulation. Thus, it appears that testosterone increases cadmium...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::438a698989a04f0f463088072eb6623d https://doi.org/10.1080/107691899229098 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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