Isolation and characterization of a poplar d-type cyclin gene associated with the SHORT-ROOT/SCARECROW network

Autor: Lei Xuan, Minren Huang, Zuoheng Wang, Sian Liu, Meng Xu
Rok vydání: 2015
Předmět:
Zdroj: Trees. 30:255-263
ISSN: 1432-2285
0931-1890
DOI: 10.1007/s00468-015-1296-y
Popis: PeCYCD6_1 , a d-type cyclin gene associated with the SHORT-ROOT/SCARECROW network during adventitious root development, was identified and isolated from poplar. SHORT-ROOT (SHR) and SCARECROW (SCR) are key regulators of root radial patterning and stele cell specification and maintenance in the Arabidopsis root. In the cortex/endodermis initial daughter cell, SHR and SCR activate the expression of a d-type cyclin, CYCD6;1, which promotes the periclinal division that maintains the separate endodermis and cortex cell layers. Information on the regulatory network is still poorly understood in trees, despite extensive and detailed analysis of the SHR/SCR regulatory network in herbaceous model organism, Here, PeCYCD6_1, a poplar d-type cycle gene was isolated from the Populus clone “Nanlin895” (Populus deltoides × Populus euramericana) and characterized. The PeCYCD6_1 gene consisted of six exons putatively encoding a polypeptide of 324 amino acids. Its mRNA levels significantly increased during adventitious root development. The putative PeCYCD6_1 protein contained two conserved domains, the cyclin_N and cyclin_C. Protoplast transient expression assays showed that a PeCYCD6_1-GFP fusion protein localized to the nucleus, and interactions between PeCYCD6_1 and PeSHR1/PeSCR were demonstrated using a biomolecular fluorescence complementation assay. These results indicated that the PeCYCD6_1 gene is involved in the SHR/SCR regulatory network regulating root patterning and growth in Populus.
Databáze: OpenAIRE
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