Cloning of a novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum

Autor:	Lanqing Ma, Hong Wang, Hechun Ye, Yanwu Guo, Zhang Hong, Yadong Yang, Yi-Jiao Ma, Benye Liu
Rok vydání:	2012
Předmět:	chemistry.chemical_classification Cloning Genetics Phylogenetic tree Intron RNA Phenylalanine Plant Science Biology Enzyme chemistry Receptor Agronomy and Crop Science Gene Biotechnology
Zdroj:	Journal of Plant Biochemistry and Biotechnology. 23:104-111
ISSN:	0974-1275 0971-7811
DOI:	10.1007/s13562-012-0184-4
Popis:	By using the TAIL-PCR and over-hang extension PCR methods, we successfully isolated a three-intron type III PKS gene and cDNA clone from the Polygonum cuspidatum Sieb. et Zucc, and named it PcPKS4. The phylogenetic analysis showed that PcPKS4 is located in a cluster containing all functionally divergent CHS-like enzymes from angiosperms. Interestingly, the CHS “gatekeeper” phenylalanine, Phe265 is uniquely replaced by Leu in PcPKS4. Different from our previously results, RNA gel–blot analysis revealed that the PcPKS4 transcripts were present at a low level in roots.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::42b9d57438902f62c7473fe0a942ff09 https://doi.org/10.1007/s13562-012-0184-4 Zobrazit plný text záznamu Full text from SpringerLink