Adsorption of Thiolated Oligonucleotides on Gold Surfaces: An Atomic Force Microscopy Study
| Autor: | and C. Naud, F. Thibaudau, N. Mourougou-Candoni |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Oligonucleotide Chemistry Atomic force microscopy Analytical chemistry Surfaces and Interfaces Activation energy Condensed Matter Physics Dissociation (chemistry) Adsorption Electrochemistry Thiol Physical chemistry Molecule General Materials Science Nanoscopic scale Spectroscopy |
| Zdroj: | Langmuir. 19:682-686 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la026021j |
| Popis: | Atomic force microscopy (AFM) was used to study oligonucleotide adsorption on a gold surface. Oligonucleotides are thiolated at the 5‘ end with SH(CH2)6-5‘ to adsorb them specifically via a gold−sulfur linkage. Thanks to nanoscopic resolution of AFM, we have been able to measure the density of adsorbed molecules, varying experimental parameters. The adsorption kinetics of the oligonucleotides shows a two-step mechanism. The first step is a nonspecific adsorption of molecules. In the second one, these oligonucleotides diffuse and react with gold. This reaction depends on the structure of the surface (steps and/or reconstructions). The rate, which increases with temperature, leads to an activation energy of thiol dissociation on gold around 100 kJ/mol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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