| Autor: |
Meenakshi Maruthamuthu, G. Selvakumar |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Proceedings / Indian Academy of Sciences. 107:79-86 |
| ISSN: |
0253-4134 |
| Popis: |
Modified Stern-Volmer equation is obeyed by bovine serum albumin (BSA)-iodide system showing selective quenching of tryptophanyl fluorescence of BSA. The fraction of accessible protein fluorescence is 0.56 and the effective Stern-Volmer constant is 290 M-1 at pH 7.4 in 0.005 M phosphate buffer at 25°C. Collisional quenching is operative both in the BSA -I−1 system and the model system, tryptophan-I−1. It is supported by the observed relationship between the ratio of quenching rate constants (k q ) and diffusion coefficients and alsok q with bulk viscosity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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