FURTHER CHARACTERIZATION OF MONOCLONAL ANTIBODY INDICATES SPECIFICITY FOR (6–4)‐DIPYRIMIDINE PHOTOPRODUCTS
| Autor: | T Matsunaga, John M. Boyle, P T Strickland, Osamu Nikaido |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
medicine.drug_class Cell Nucleic acid sequence Pyrimidine dimer General Medicine Monoclonal antibody Biochemistry Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry medicine biology.protein Physical and Theoretical Chemistry Antibody Photolyase DNA Action spectrum |
| Zdroj: | Photochemistry and Photobiology. 55:723-727 |
| ISSN: | 1751-1097 0031-8655 |
| Popis: | — Monoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol.34, 595–601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent tight. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6–4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings. |
| Databáze: | OpenAIRE |
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