ChemInform Abstract: An Enzymatic Method for Preparation of Homopolymannuronate Blocks and Strictly Alternating Sequences of Mannuronic and Guluronic Units

Autor: Micheline Guinand, Alain Heyraud, Frederic Chavagnat, Jean Wallach, Philippe Colin-Morel, Claude Gey
Rok vydání: 2010
Předmět:
Zdroj: ChemInform. 29
ISSN: 1522-2667
0931-7597
Popis: Two Pseudomonas aeruginosa alginates were lysed by an overexpressed polymannuronate lyase AlxM B (only acting on two or more consecutive, nonacetylated mannuronate units) to prepare either mannuronate blocks (poly-M blocks) with dp∼30, or strictly alternating sequences of mannuronic and guluronic acid (poly-MG blocks) with dp>20. The poly-M blocks were obtained by lysis of a P. aeruginosa polymannuronate that has 50% O-acetylation at C-2 and C-3. The poly-MG blocks were obtained from a P. aeruginosa alginate that contained both mannuronate and guluronate residues. The polysaccharide was first deacetylated and then treated with the lyase to excise the mannuronate units from the alternating-MG blocks. Both types of blocks should have potent biological effects and should provide useful specific substrates for characterisation of other alginate lyases.
Databáze: OpenAIRE
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