Effectiveness of Sodium Borohydride Treatment on Acid Hydrolyzates from Olive-Tree Pruning Biomass for Bioethanol Production
| Autor: | Alberto J. Moya, Silvia Peinado, Sebastián Sánchez, Soledad Mateo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Chromatography biology Renewable Energy Sustainability and the Environment 020209 energy Oxalic acid 02 engineering and technology Xylose biology.organism_classification 01 natural sciences chemistry.chemical_compound Acetic acid Hydrolysis Sodium borohydride chemistry 010608 biotechnology 0202 electrical engineering electronic engineering information engineering Ethanol fuel Fermentation Pichia stipitis Agronomy and Crop Science Energy (miscellaneous) |
| Zdroj: | BioEnergy Research. 12:302-311 |
| ISSN: | 1939-1242 1939-1234 |
| Popis: | The application of an effective removal treatment to minimize inhibitory compounds, present after hydrolysis treatment, might mean a great advance on renewable biofuel generation strategy since growth-inhibiting substances, such as phenolic compounds and furans, are one of the major problems in the development of fermentative step. Detoxification of olive-tree pruning hydrolyzates using NaBH4, as reducing agent, has had a positive effect on fermentative parameters using Pichia stipitis CBS 6054. Oxalic acid treatment conditions have been optimized (150 ∘C and 75 mM of oxalic acid in order to obtain the maximum fermentable sugar recovery without an excessive inhibitory compounds production) considering the combined severity factor, CSF, (CSF = 1.56 ± 0.05). Hydrolyzate characterization at optimal condition showed concentrations (in g/L) of 16.64 D-glucose, 15.05 D-xylose, 5.16 L-arabinose, 2.87 acetic acid, 3.18 phenolic compounds, and 0.96 furans. Sodium borohydride conditioning process has been studied using a response surface methodology. The use of NaBH4 (0.03 mol/L during 30 min at pH= 6.0) has been demonstrated to be a successful conditioning method for almost entirely furans elimination as well as 40% of phenolic compounds reduction. As a result, it has been possible to ferment sugary liquors which did not ferment without such treatment, obtaining a maximum ethanol yield of 27% (3.8 g/L) in treated hydrolyzates under optimum conditions. |
| Databáze: | OpenAIRE |
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