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AIM: To investigate the inhibitory effects of SS1 and NCTC11637 antigens on the proliferation of Hela cells, and to observe the neutralization of bovine anti-Helicobacter pylori antibodies against SS1 and NCTC11637. METHODS: The cows were immunized with sonicated Helicobacter pylori (H pylori) antigen. After the scheduled immunization was completed, the immunization was strengthened with an interval of 1-2 month.The bovine se- rum was prepared when the ratio in agar-double diffusion was 1 32. The bovine milk was collected at the same time. The serum proteins (81.4g/L) were isolated and pu- rified by saturated ammonium sulfate (500, 330, 330 g/L) and DEAE-32. The obtained proteins were identified by SDS-PAGE. Bovine anti-H pylori IgGs were incubated with SS1 or NCTC11637, respectively, at 37 for 2 h, and then added into the solutions including Hela cells. The proliferation of Hela cells was determined by cell growth curve. MTT assay was adopted to evaluate the inhibitory effects of SS1 and NCTC11637 antigens on the prolifera- tion of Hela cells. RESULTS: The bacterial protein of SS1 and NCTC11637 inhibited the proliferation of Hela cells in a concentration- dependent manner (SS1: r = 0.9 594; NCTC11637: r = 0.9 371), and the cells manifested apoptotic or lysed features. For the same effect, a higher concentration of SS1 was needed. After treatment with bovine anti-H pylori IgG, the inhibitory effects of SS1 and NCTC11637 on Hela cells were neutralized, and their inhibition rates were nega- tively related with the concentration of bovine anti-H pylori IgG (SS1: r = -0.9 936; NCTC11637: r = -0.9 627). CONCLUSION: SS1 and NCTC11637 can inhibit the pro- liferation of Hela cells, and bovine anti-H pylori antibodies can neutralize the inhibiting action in a concentration-de- pendent manner. |
