The master transcription factor Spo0A is required for poly(3-hydroxybutyrate) (PHB) accumulation and expression of genes involved in PHB biosynthesis in Bacillus thuringiensis
| Autor: | Shih-Chuan Pan, Hui-Ju Chen, Chi-Ling Tseng, Jer-Sheng Lin, Teng-Kuan Tsai, Gwo-Chyuan Shaw |
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| Rok vydání: | 2010 |
| Předmět: |
Regulation of gene expression
fungi technology industry and agriculture macromolecular substances Biology biology.organism_classification Microbiology Cell biology Complementation Transcription (biology) Bacillus thuringiensis Gene expression Genetics bacteria lipids (amino acids peptides and proteins) Molecular Biology Transcription factor Gene Regulator gene |
| Zdroj: | FEMS Microbiology Letters. 304:74-81 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2009.01888.x |
| Popis: | Bacillus thuringiensis is a gram-positive spore-forming bacterium that can accumulate poly(3-hydroxybutyrate) (PHB) as a carbon and energy storage substance in response to nutritional stress. The regulatory mechanism for PHB biosynthesis in B. thuringiensis and diverse Bacillus species is still poorly understood. We now report that disruption of the sigH gene or the gene encoding the master sporulation transcription factor Spo0A severely impaired PHB accumulation in B. thuringiensis. Complementation of the spo0A mutation with the spo0A gene restored PHB accumulation. We have found that the requirement of Spo0A for PHB accumulation is independent of the transition state regulator AbrB and of loss of sporulation ability. We also show that Spo0A is required for the expression of three genes involved in PHB biosynthesis. These findings have uncovered a new role of Spo0A in the regulation of stationary-phase-associated cellular events. |
| Databáze: | OpenAIRE |
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