The role of hyaluronate in morphogenesis of the neurons

Autor: I. R. Nikonenko, Galina Skibo, E. A. Lepekhin, M. Witt, G. A. Ushakova
Rok vydání: 1997
Předmět:
Zdroj: Neurophysiology. 29:16-21
ISSN: 1573-9007
0090-2977
DOI: 10.1007/bf02463181
Popis: The data about organization of the extracellular matrix (ECM) components and their interplay in the mammalian brain are rather limited. Hyaluronate (HA) is one of the main ECM glycosaminoglycans. Its location and function in the brain are believed to be mediated through its interaction with HA-binding proteins and proteoglycans. In this report, we describe distribution of the total HA-binding activity in the cells in the course of postnatal development of the rat brain and the effect of HA on cultured neurons. A high level of the HA-binding activity was found in the newborn cerebellum, but it quickly decreased after postnatal day 1. On postnatal day 5, strong HA-binding activity was demonstrated only in apical parts of growth cones of Purkinje cells. The data showed rapid down-regulation of HA-binding activity at the first stage of cerebellum maturation (migration of granule cells and beginning of differentiation of neurons). To obtain more information concerning a key role of HA in morphogenesis of neurons, low density cell cultures of the hippocampal neurons were used. The presence of HA in the substrate led to an increase in the cell adherence. However, a part of the cells got differentiated later. These data allow us to suggest that interactions between extracellular HA and cell-surface receptors can regulate motility and differentiation of the neurons.
Databáze: OpenAIRE
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