Cytocompatibility of Porous Biphasic Calcium Phosphate Granules With Human Mesenchymal Cells by a Multiparametric Assay
| Autor: | Bruno König, Fabio Franceschini Mitri, Gutemberg Gomes Alves, Alexandre J R Rossi, José Mauro Granjeiro, Gustavo Vicentis de Oliveira Fernandes |
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| Rok vydání: | 2012 |
| Předmět: |
Neutral red
Biocompatibility Mesenchymal stem cell Biomedical Engineering Medicine (miscellaneous) chemistry.chemical_element Bioengineering General Medicine Calcium Biomaterials chemistry.chemical_compound chemistry Tissue engineering Viability assay Crystal violet Bone regeneration Biomedical engineering Nuclear chemistry |
| Zdroj: | Artificial Organs. 36:535-542 |
| ISSN: | 0160-564X |
| DOI: | 10.1111/j.1525-1594.2011.01409.x |
| Popis: | This work aims to evaluate the cytocompatibility of injectable and moldable restorative biomaterials based on granules of dense or porous biphasic calcium phos- phates (BCPs) with human primary mesenchymal cells, in order to validate them as tools for stem cell-induced bone regeneration. Porous hydroxyapatite (HA) and HA/beta- tricalcium phosphate (b-TCP) (60:40) granules were obtained by the addition of wax spheres and pressing at 20 MPa,while dense materials were compacted by pressing at 100 MPa,followed by thermal treatment (1100°C),grind- ing,and sieving.Extracts were prepared by 24-h incubation of granules on culture media,with subsequent exposition of human primary mesenchymal cells. Three different cell viability parameters were evaluated on the same samples. Scanning electron microscopy analysis of the granules revealed distinct dense and porous surfaces. After cell exposition to extracts, no significant differences on mitochondrial activity (2,3-bis(2-methoxy-4-nitro-5- sulfophenly)-5-((phenylamino) carbonyl)-2H-tetrazolium hydroxide) or cell density (Crystal Violet Dye Elution) were observed among groups.However,Neutral Red assay revealed that dense materials extracts induced lower levels of total viable cells to porous HA/b-TCP (P < 0.01). Calcium ion content was also significantly lower on the extracts of dense samples. Porogenic treatments on BCP composites do not affect cytocompatibility, as measured by three different parameters, indicating that these ceramics are well suited for further studies on future bioengineering applications. Key Words: Stem cells—Biocompatibility —Biphasic calcium phosphate—Tissue engineering. |
| Databáze: | OpenAIRE |
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