Human immunodeficiency virus type-1 (HIV-1) genetic diversity and prevalence of antiretroviral drug resistance mutations in treatment-nave adults in Jos, North Central Nigeria
| Autor: | J A Anejo Okopi, O O Agbaji, P A Agaba, P O Ugoagwu, K Were, H Onywera, P Owiti, S E Isa, N Otecko, A E J Okwori, J Musa, S Oguche, A S Sagay, J A Idoko, L Nimzing, E D Jatau, null Olonitola, S O |
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| Rok vydání: | 2013 |
| Předmět: |
Mutation
Genetic diversity business.industry virus diseases Context (language use) Drug resistance Resistance mutation medicine.disease_cause Vaccine efficacy Applied Microbiology and Biotechnology Virology Reverse transcriptase Subtyping Genetics Medicine business Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology. 12:2279-2287 |
| ISSN: | 1684-5315 2279-2287 |
| DOI: | 10.5897/ajb2013.11954 |
| Popis: | The presence of human immunodeficiency virus (HIV) type-1 diversity has an impact on vaccine efficacy and drug resistance. It is important to know the circulating genetic variants and associated drug-resistance mutations in the context of scale up of antiretroviral therapy (ART) in Nigeria. The objective of this study was to determine the genetic diversity of HIV-1 and the prevalence of antiretroviral (ARV) drug resistance mutations among antiretroviral treatment-naive HIV-1 infected patients in Jos, North Central Nigeria. Plasma samples were collected from 105 ARV drug-naive patients enrolled for HIV care at the Jos University Teaching Hospital (JUTH) HIV Treatment Center between October 2010 and April 2011. One hundred (100) samples were successfully amplified. Viral subtyping was done using REGA subtyping tool and by phylogenetic analysis using PAUP software. The drug resistance mutations were determined using the Stanford University HIVdb sequence interpretation algorithm. HIV-1 subtypes identified were; CRF02_AG (48.0%), G (41.0%), CRF06_cpx (6.0%) and A1 (5.0%). 8% of the patients’ isolates had at least one major resistance mutation in the RT gene: Nucleoside reverse transcriptase inhibitors: M41L (1%), K65KR (1%), M184IM (1%), M184V (2%) and T215ADNT (1%), non-nucleoside reverse transcriptase inhibitors: K103N (2%), K101E (1%), G190A (1%), P225HP (1%), Y181I (1%), Y188L (1%), and Y181C (1%). Among antiretroviral (ARV) naive patients in Jos, North Central Nigeria, the common HIV-1 subtypes was CRF_02 and G. And the prevalence of drug resistance mutations was found to be high (8%). Further study and national surveillance will be critically important to understand the clinical impact of transmitted resistance mutations on ART naive individuals in resource limited settings. Keywords : HIV-1 subtypes, antiretroviral (ARV), treatment-naive, drug-resistance, mutation, accessory and polymorphisms, Nigeria African Journal of Biotechnology Vol. 12(17), pp. 2279-2287 |
| Databáze: | OpenAIRE |
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