Improved separation of basic peptides in anion-exchange chromatography

Autor:	Lars Strid, Per Olof Nyman, Betty M. Malmström
Rok vydání:	1981
Předmět:	Solvent system Chromatography Resolution (mass spectrometry) Arginine Ion exchange Chemistry Organic Chemistry Lysine General Medicine Biochemistry Analytical Chemistry
Zdroj:	Journal of Chromatography A. 215:109-114
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(00)81391-6
Popis:	A method is described for the separation of peptides on anion-exchange-resin columns with particular emphasis being given to the resolution of basic arginine and basic lysine peptides. The addition of N-methylpiperidine, p K a 10.08, to the solvent systems used either with stepwise or gradient separations improves the resolution in the high pH range compared to methods previously used.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3dd3ebd93e8063e387e63cc29cf1a861 https://doi.org/10.1016/s0021-9673(00)81391-6 Zobrazit plný text záznamu