Construction of an off-line two dimensional reversed-phase liquid chromatography/ultra-high performance supercritical fluid chromatography method for rapid and comprehensive analysis of Piper kadsura
| Autor: | Yu Jin, Yanxiong Ke, Yun Yuan, Yanfang Liu, Xinmiao Liang, Huaxia Xin, Qing Fu |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
biology General Chemical Engineering 010401 analytical chemistry Ethyl acetate Analytical chemistry Fraction (chemistry) 02 engineering and technology Reversed-phase chromatography 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Countercurrent chromatography Two-dimensional chromatography chemistry Piper kadsura Supercritical fluid chromatography Physical and Theoretical Chemistry 0210 nano-technology Chromatography column |
| Zdroj: | The Journal of Supercritical Fluids. 127:9-14 |
| ISSN: | 0896-8446 |
| DOI: | 10.1016/j.supflu.2017.03.004 |
| Popis: | In this study, ultra-high performance supercritical fluid chromatography (UHPSFC) was chosen to construct an off-line two dimensional chromatography with reversed-phase liquid chromatography (RPLC) for rapid and comprehensive analysis of Piper kadsura. The sample was firstly divided into a petroleum ether (PE) extract and an ethyl acetate (EA) extract, and then they were separated into 21 and 28 fractions in the first dimensional RPLC, respectively. In the second dimension, the effect of UHPSFC parameters on the analysis speed was investigated. Each PE fraction could be analyzed in 2.0 min, and every EA fraction could be analyzed in 3.0 min. In total, at least 1033 peaks were detected by this method, including 204 peaks from the PE fractions and 829 peaks from the EA fractions. The 2D-RPLC/UHPSFC system with the characteristics of fast separation and high orthogonality has a great potential for the rapid and comprehensive analysis of the complex samples. |
| Databáze: | OpenAIRE |
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