| Popis: |
This study was to develop the entrapment model of various charged peptide drugs (bacitracin, insulin and bovine serum albumin(BSA)) in nanovesicles (liposomes and niosomes). Cationic, anionic and neutral liposomes were prepared from dipalmitoyl phosphatidylcholine (DPPC), cholesterol (CHL), dimethyldioctadecylammonium bromide (DDAB) and/or dicetylphosphate (DP), whereas cationic, anionic and neutral niosomes were composed of sorbitan monostearate (Span 60) or polyoxyethylene sorbitanmonostearate (Tween 61), CHL, DDAB and/or DP at various molar ratios. Morphology and vesicular sizes of the vesicles were investigated by optical microscope and dynamic light scattering (DLS), respectively. The entrapment efficiency of the peptides in the vesicles was determined by gelelectrophoresis and gel documentation. The prepared empty liposomes and niosomes were in white powder after lyophilization. They were multilamellar layers with the vesicular sizes ranging from 40-60 nm, except for the neutral niosomes entrapped with insulin and cationic niosomes entrapped with BSA showed the sizes ranging from 0.1-1.3 μm. and 100-150 nm, respectively. The entrapment efficiency of BSA, bacitracin and insulin in neutral niosomes (Tween61/CHL 1:1) was 72.9%, 69.9% and 10.3%, in cationic niosomes (Tween61/CHL/DDAB 1:1:0.05) was 84.5%, 32.8 and 87.2% and in anionic niosomes (Tween61/CHL/DP 1:1:0.05) was 50.1%, 90.9% and 44.3%, respectively. This study has demonstrated the enhancement of entrapment efficiency of various peptide drugs by modifying the charges of the bilayer vesicles. The results from this study can suggest the proper model of nanovesicular formulation to entrap peptides with different charges and polarity for pharmaceutical and cosmeceutical application. |
