| Autor: |
E.Yu. Misionzhnik, Yu. A. Gryzunov, A.V Molodetskich, M.N. Yakimenko, M. N. Komarova, G. E. Dobretsov, T. Syrejshchikova, M. G. Uzbekov |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 448:478-482 |
| ISSN: |
0168-9002 |
| Popis: |
The properties of serum albumin obtained from donors and from paranoid schizophrenia patients were studied with the fluorescent probe K-35 (N-carboxyphenylimide of dimethylaminonaphthalic acid) and time-resolved fluorescence spectroscopy on the SR beam station of the S-60 synchrotron of the Lebedev Physical Institute. The mean fluorescence quantum yield of K-35 in patients serum was decreased significantly by 25–60% comparing with donors. The analysis of pre-exponential factors of fluorescence decay using “amplitude standard” method has shown that in patient sera the fraction of K-35 molecules bound with albumin and inaccessible to fluorescence quenchers (“bright” K-35 molecules with τ 1 =8.0±0.4 ns) is 1.2–3 times less than in the donor sera. The fraction of K-35 molecules with partly quenched fluorescence ( τ 2 =1.44±0.22 ns) was significantly increased in schizophrenia patients. The results obtained suggest that the properties of binding region in serum albumin molecules of acute paranoid schizophrenia patients change significantly. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect