Water avoidance stress results in an altered voiding phenotype in male mice
| Autor: | Stephen A. Zderic, Douglas A. Canning, Erin McGonagle, Rita J. Valentino, Ariana L. Smith, Stephan Butler, Joanna Sliwoski |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test business.industry Urology media_common.quotation_subject Cystometry NFAT Urination Muscle hypertrophy Bladder outlet obstruction Endocrinology Hypoxia-inducible factors In vivo Internal medicine Myosin medicine Neurology (clinical) business media_common |
| Zdroj: | Neurourology and Urodynamics. 31:1185-1189 |
| ISSN: | 0733-2467 |
| Popis: | Aims We set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO). Methods Six-week-old male Swiss Webster mice housed with sibling littermates were individually placed on a platform centered in the middle of a water filled basin for 1 hr daily for 4 weeks. A non stressed cohort of sibling littermates served as controls. Measured end points included voiding frequency, voided volume, bladder mass, and in vivo cystometry. Molecular end points included myosin heavy chain (MHC) isoform distribution by PCR, and nuclear translocation of hypoxia inducible factor (HIF1α) and the nuclear factor of activated T-cells (NFAT) by gel shift assay. These molecular endpoints were compared with samples from male mice undergoing anatomic pBOO. Results WAS resulted in increased average voided volumes and bladder mass, and a decrease in voiding frequency (P |
| Databáze: | OpenAIRE |
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