Peptides isolation from crude somatic antigens of Haemonchus contortus through SDS- PAGE

Autor:	Muhammad Imran, Muhammad Sohail Sajid, Asim Shamim, Muhammad Nisar Khan, Muhammad Saqib
Rok vydání:	2017
Předmět:	General Veterinary biology Somatic cell biology.organism_classification Isolation (microbiology) Molecular biology Microbiology Antigen Immunity parasitic diseases Animal Science and Zoology Gastrointestinal nematode Bradford protein assay Polyacrylamide gel electrophoresis Haemonchus contortus
Zdroj:	Indian Journal of Animal Research.
ISSN:	0976-0555 0367-6722
DOI:	10.18805/ijar.v0iof.8473
Popis:	Haemonchus (H.) contortus is a blood feeding gastrointestinal nematode, and is considered one of the major threats for goat health and production globally. Crude somatic antigens (CSA) have been reported as sources of immunogens and provide better level of immunity against gastrointestinal nematodes. The present study aimed to quantify CSA of H. contortus worms (n=25) of both sexes followed by isolation of peptides through Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and their characterization. The triturate contained proteins having concentration of 1.3mg/ml as measured through Bradford assay. Seven different bands were appeared on gel ranging from 35KDa to 170KDa. The future prospects may include identification of the immunogenic peptides which can be used as vaccine candidates against H. contortus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3b2c94081b96af7634fd0fc20e794f61 https://doi.org/10.18805/ijar.v0iof.8473 Zobrazit plný text záznamu