Developmental Changes in NO Synthase-Containing Sensory Neurons in Chemical Deafferentation with Capsaicin

Autor: K. Yu. Moiseev, V. V. Porseva, V. P. Smirnova, M. B. Korzina, P. M. Maslyukov
Rok vydání: 2015
Předmět:
Zdroj: Neuroscience and Behavioral Physiology. 45:991-995
ISSN: 1573-899X
0097-0549
DOI: 10.1007/s11055-015-0176-7
Popis: Immunohistochemical and morphometric methods were used to determine the locations, relative contents, and morphometric characteristics of neurons expressing NO synthase (NOS) in the caudal ganglion of the vagus nerve (CGVN) and spinal nerve sensory ganglia (SNSG) in female Wistar rats (n = 25) aged 3, 10, 20, 30, and 60 days subjected to chemical deafferentation on the second day of life by administration of single doses of capsaicin. The control group consisted of rats (n = 25) of the same ages. The results obtained from these studies provided evidence that in the control group, the proportion of NOS-immunopositive neurons in the SNSG increased during the first 10 days of life and decreased from day 30 to day 60. In the CGVN, the proportion of NOS-immunopositive neurons did not show any significant change during ontogeny. In experimental animals, the proportions of NOS-positive neurons in the SNSG and CGVN decreased sharply during the first 20 days of life. The decrease in the number of NOS-containing neurons in the SNSG was more marked than that in the CGVN. These data provide evidence of the harmful effect of capsaicin on NOS-positive neurons, supporting the role of NO in the mechanisms of nociception.
Databáze: OpenAIRE
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