Expression of the T-box family genes,Tbx1-Tbx5, during early mouse development

Autor: Jeremy J. Gibson-Brown, Judith A. Cebra-Thomas, Lee M. Silver, Virginia E. Papaioannou, Deborah L. Chapman, Sarah Hancock, Maria Alexiou, Roni J. Bollag, Nancy Garvey, Sergei I. Agulnik
Rok vydání: 1996
Předmět:
Zdroj: Developmental Dynamics. 206:379-390
ISSN: 1097-0177
1058-8388
DOI: 10.1002/(sici)1097-0177(199608)206:4<379::aid-aja4>3.0.co;2-f
Popis: A novel family of genes, charac- terized by the presence of a region of homology to the DNA-binding domain of the Brachyury (2') lo- cus product, has recently been identified. The re- gion of homology has been named the T-box, and the new mouse genes that contain the T-box do- main have been named T-box 1-6 (Tbxl through TbxG). As the basis for further study of the func- tion and evolution of these genes, we have exam- ined the expression of 5 of these genes, Tbxl- Tbx5, across a wide range of embryonic stages from blastocyst through gastrulation and early or- ganogenesis by in situ hybridization of whole- mounts and tissue sections. Tbx3 is expressed ear- liest, in the inner cell mass of the blastocyst. Four of the genes are expressed in different compo- nents of the mesoderm or mesodedendoderm during gastrulation (Tbxl and Tbx3-5). All of these genes have highly specific patterns of ex- pression during later embryogenesis, notably in areas undergoing inductive tissue interactions. In several cases there is complementary expression of different genes in 2 interacting tissues, as in the lung epithelium (Tbxl) and lung mesenchyme (Tbx2-5), and in mammary buds (Tbx3) and mam- mary stroma (Tbx2). Tbxl shows very little over- lap in the sites of expression with the other 4 genes, in contrast to a striking similarity in ex- pression between members of the 2 cognate gene sets, Tbx21Tbx3 and Tbx4lTbx5. This is a clear re- flection of the evolutionary relationship between the 5 genes since the divergence of Tbxl occurred long before the relatively recent divergence of Tbx2 and 3 and Tbx4 and 5 from common ances- tral genes. These studies are a good indication that the T-box family of genes has important roles in inductive interactions in many stages of mam- malian embryogenesis.
Databáze: OpenAIRE
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