Analysis of a live attenuated bacterial vaccine for tularemia in the MIMIC® (Modular Immune In vitro Construct) System (52.6)
| Autor: | Janice Moser, Emily Sassano, Christopher Muerrle, David Waag, Vaughan Wittman |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 186:52.6-52.6 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.186.supp.52.6 |
| Popis: | Sanofi Pasteur has optimized a series of unique, sensitive, automatable, and cost-effective assay systems, termed the Modular IMmune In vitro Construct (MIMIC®), to permit the evaluation of human innate and adaptive immunity in a laboratory setting. The lymphoid tissue equivalent (LTE) module of the MIMIC® platform reproduces the correct cellular interactions and kinetics to initiate primary (naïve) human B and T cell responses in an in vitro setting. We are currently investigating LVS, a live attenuated bacterial vaccine strain of Francisella tularensis, in the MIMIC® System. LVS generates a strong, naïve, TH1-skewed CD4 T cell response demonstrated by the percentage of responding cells producing intracellular IFNγ and TNFα. This data is consistent with human in vivo data showing that protection elicited by LVS vaccine includes a strong cellular immunity component. Previous studies have also shown that LVS drives production of protective antibodies in humans. We have currently demonstrated that the MIMIC® System can drive the generation of LVS-specific antibody responses in vitro using a fully autologous system. These studies indicate that the in vitro MIMIC® system offers a platform in which to conduct experiments in a fully human system prior to the involvement of human subjects. Therefore, the MIMIC® system provides a platform for analysis of new vaccine candidates and can be utilized for greater understanding of how efficient vaccines function. |
| Databáze: | OpenAIRE |
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