Androgen Receptor Gene Tagging Single Nucleotide Polymorphisms are not Associated with Polycystic Ovary Syndrome in South Indian Women

Autor:	Perumal Venkatachalam, Sanjeeva Reddy Nellepalli, Solomon F. D. Paul, Himavanth Reddy Kambalachenu
Rok vydání:	2014
Předmět:	Androgen receptor medicine.medical_specialty Endocrinology Internal medicine Haplotype medicine SNP Population study Single-nucleotide polymorphism Biology Restriction fragment length polymorphism Genotyping Polycystic ovary
Zdroj:	IOSR Journal of Pharmacy and Biological Sciences. 9:07-11
ISSN:	2278-3008 2319-7676
DOI:	10.9790/3008-09450711
Popis:	Impaired steroidogenesis at gonads or defect in the receptors mediating the actions of those hormones is being reported as an etiological factor for Polycystic ovary syndrome (PCOS). Of the various factors, variations in the expression of androgen receptor (AR) gene have been shown as a risk factor for women with PCOS; however the findings were not consistent among populations studied. Therefore, the present study was aimed to screen the prevalence of AR gene haplotype tagging single nucleotide polymorphisms (SNP) and its association with PCOS in south Indian women. The study population consists of a total of 198 subjects, of which 97 were women with PCOS and 101 healthy women as controls. Genotyping of SNPs for AR rs5919393 (C/T), rs5919411 (A/G) and rs12014709 (G/T) were carried out by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and DNA sequencing. Out of three SNPs examined, the rs5919393 (C/T) and rs5919411 (A/G) are monomorphic in both PCOS and controls. Whereas, rs12014709 (G/T) was observed
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::38dc55a7d1a68c15eee2d5c2c60f94df https://doi.org/10.9790/3008-09450711 Zobrazit plný text záznamu