| Autor: |
Anmol Krishna, Tyler J. Donner, Jason Gardiner, Enrico Scarpella |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.09.28.317644 |
| Popis: |
Activation of gene expression in striped domains is a key building block of biological patterning, from the recursive formation of veins in plant leaves to that of ribs and vertebrae in our bodies. In animals, gene expression is activated in striped domains by the differential affinity of broadly expressed transcription factors for their target genes and the combinatorial interaction between such target genes. In plants, how gene expression is activated in striped domains is instead unknown. We address this question for the broadly expressed MONOPTEROS (MP) transcription factor and its target geneARABIDOPSIS THALIANA HOMEOBOX FACTOR8(ATHB8). We find thatATHB8promotes vein formation and that such vein-forming function depends on both levels ofATHB8expression and width ofATHB8expression domains. We further find thatATHB8expression is activated in striped domains by a combination of (1) activation ofATHB8expression through binding of peak levels of MP to a low-affinity MP-binding site in theATHB8promoter and (2) repression ofATHB8expression by MP target genes of theINDOLE-3-ACETIC-ACID-INDUCIBLEfamily such asBODENLOS. Our findings suggest that a common regulatory logic controls activation of gene expression in striped domains in both plants and animals despite the independent evolution of their multicellularity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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