Interaction of fatty acids with lipid bilayers
| Autor: | E.K. Rooney, Anthony G. Lee, J M McWhirter, J M East, A.C. Simmonds, O.T. Jones |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
chemistry.chemical_classification
Quenching (fluorescence) Chromatography Biophysics Phospholipid Fatty acid Cell Biology Biochemistry Fluorescence spectroscopy chemistry.chemical_compound Oleic acid chemistry Phospholipid Binding lipids (amino acids peptides and proteins) Stearic acid Lipid bilayer |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 728:159-170 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(83)90467-4 |
| Popis: | We present a method by which it is possible to describe the binding of fatty acids to phospholipid bilayers. Binding constants for oleic acid and a number of fatty acids used as spectroscopic probes are deduced from electrophoresis measurements. There is a large shift in p K value for the fatty acids on binding to the phospholipid bilayers, consistent with stronger binding of the uncharged form of the fatty acid. For dansylundecanoic acid, fluorescence titrations are consistent with the binding constants derived from the electrophoresis experiments. For 12-(9-anthroyloxy)stearic acid, fluorescence and electrophoresis data are inconsistent, and we attribute this to quenching of fluorescence at high molar ratios of 12-anthroylstearic acid to phospholipid in the bilayer. |
| Databáze: | OpenAIRE |
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