Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design

Autor:	Fang-Qiu Guo, Huo-fei Zhou, Qiu-ju Jiang, Lan-Fang Huang
Rok vydání:	2006
Předmět:	Peak area Chromatography Materials science Mechanical Engineering Extraction (chemistry) Analytical chemistry High-performance liquid chromatography Solvent Surface-area-to-volume ratio Mechanics of Materials Uniform design General Materials Science Radix Hplc dad
Zdroj:	Journal of Central South University of Technology. 13:156-159
ISSN:	1993-0666 1005-9784
DOI:	10.1007/s11771-006-0148-8
Popis:	Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography (HPLC) at detection wavelength of 320 nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85°C and a volume ratio of solvent to sample of 10 : 1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3658c7d80f73c1f7835ad7180dc4d765 https://doi.org/10.1007/s11771-006-0148-8 Zobrazit plný text záznamu Full text from SpringerLink