Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy
| Autor: | Angshumonik Angbohang, P J Banerjee, Karen Eastlake, Peng T. Khaw, DG Charteris, Ga Limb |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Retina Proliferative vitreoretinopathy Pathology medicine.medical_specialty medicine.medical_treatment Retinal Biology medicine.disease 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure Cytokine Neurology chemistry Downregulation and upregulation Gliosis Cell culture Immunology medicine sense organs medicine.symptom Muller glia |
| Zdroj: | Glia. 64:495-506 |
| ISSN: | 0894-1491 |
| DOI: | 10.1002/glia.22942 |
| Popis: | Retinal gliosis is characterized by biochemical and physiological changes that often lead to Muller glia proliferation and hypertrophy and is a feature of many neuro-degenerative and inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although Muller glia are known to release inflammatory factors and cytokines, it is not clear whether cytokine production by these cells mirrors the pattern of factors present in the gliotic retina. Lysates from normal cadaveric retina and gliotic retinal specimens from patients undergoing retinectomy for treatment of PVR, the Muller cell line MIO-M1 and four human Muller glial cell preparations isolated from normal retina were examined for their expression of cytokines and inflammatory factors using semi-quantitative dot blot antibody arrays and quantitative arrays. Comparative analysis of the expression of inflammatory factors showed that in comparison with normal retina, gliotic retina exhibited greater than twofold increase in 24/102 factors examined by semiquantitative arrays, and a significant increase in 19 out of 27 factors assessed by quantitative methods (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text