Influence of Green (Brazilian) propolis on the expression levels of miRNAs related to oxidative stress and inflammation
| Autor: | Di Lorenzo, Arianna, Zaccaria, Vincenzo, Curti, Valeria, Fachini, Alfredo, Daglia, Maria |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| DOI: | 10.18143/jisanh_v3i4_1394 |
| Popis: | Propolis is a natural substance collected by honey bees from different plants. A large body of evidence showed that propolis exerts many physiological and pharmacological properties such as antimicrobial (antibacterial, antiviral and antifungal activities), antioxidant, and anti-inflammatory properties. The mechanisms of action at the basis of propolis capacity to counteract oxidative stress and inflammation has not been yet elucidated. Thus, the aim of this study is to show that a high quality Green Brazilian propolis extract (prepared according to a patented technology) exerts epigenetic effects, being able to modulate the expression levels of miRNAs connected with oxidative stress and inflammation. The epigenetic effect of green propolis was studied by evaluating the expression levels of two miRNAs, miR-27a-3p and miR-19a-3p, in cells HaCat (human keratinocytes), treated with growing concentrations of green propolis tested at sub-toxic concentrations. Moreover, the study of the expression levels of mRNAs coding for NFE2L2 and TNF-α, validated target of miR-27a-3p and miR-19a-3p respectively, revealed a decreased in agreement with the increase of the corresponding miRNA. Thus, we can conclude that propolis exerts its anti-oxidant and antinflammatory effects through an epigenetic mechanism. Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 4 (2016) |
| Databáze: | OpenAIRE |
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